flhO

168

flagellar basal-body rod protein, required for hook assembly

Locus: BSU_36400

Molecular weight: 29.28 kDa

Isoelectric point: 4.73

Protein length: 270 aa

Gene length: 813 bp

Function: motility and chemotaxis

Product: flagellar basal-body rod protein

Essential: no

Synonyms: flhO, flgG, yvyA

Genomic Context

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List of homologs in different organisms, belongs to COG4786 (Galperin et al., 2021)

Gene

Coordinates: 3,746,279  3,747,091

Phenotypes of a mutant

• lack of SigD-dependent gene expression (no ''hag'' expression), no motility  PubMed
• defective in Swarming and flagellar assembly PubMed

The protein

Catalyzed reaction/ biological activity

• assembly of the flagellar hook PubMed
• part of the flagellar rod-hook (in FliE-FlgB-FlgC-FlhO-FlhP-FlgE out, connection to Hag) PubMed

Protein family

• Flagella basal body rod proteins family (according to UniProt)

Structure

• 5JXL (PDB) (from Campylobacter jejuni, 30% identity) PubMed
• P39752 (AlphaFold)

Paralogous protein(s)

• FlgE, FlhP

Localization

• extracellular (no signal peptide) PubMed
• flagellum hook (according to UniProt)

Expression and Regulation

Operons

• Genes: flhO-flhP

  Description: PubMed

  Sigma factors

  • SigD: sigma factor, PubMed, in sigD regulon

Biological materials

Mutant

• BKE36400 (flhO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATTTCCCACCTCTAAAA,  downstream forward: _UP4_TAAGAAAACGGAAGAAAAAA
• BKK36400 (flhO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATTTCCCACCTCTAAAA,  downstream forward: _UP4_TAAGAAAACGGAAGAAAAAA

References

Reviews

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Original Publications

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