comEB

168

dCMP deaminase, late competence protein, nucleotide scavenging protein for efficient reutilization of the products of degradation of the non-transforming strand during DNA uptake

Locus: BSU_25580

Molecular weight: 20.82 kDa

Isoelectric point: 5.37

Protein length: 189 aa

Gene length: 570 bp

Function: nucleotide scavenging

Product: dCMP deaminase

Essential: no

Synonyms: comEB, comD

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2131 (Galperin et al., 2021)

Gene

Coordinates: 2,639,877  2,640,446

The protein

Catalyzed reaction/ biological activity

• deoxycytidylate monophosphate (dCMP) deaminase PubMed
• nucleotide scavenging protein for efficient reutilization of the products of degradation of the non-transforming strand during DNA uptake PubMed

Protein family

• Cytidine and deoxycytidylate deaminase family (according to UniProt)

Domains

• CMP/dCMP-type deaminase domain (aa 5–132) (according to UniProt)

Structure

• 2HVV (PDB) (from Streptococcus mutans, 50% identity) PubMed
• P32393 (AlphaFold)

Localization

• localizes to one or both cell poles PubMed

Expression and Regulation

Operons

• Genes: comEA-comEB-comEC

  Description: PubMed

  Regulatory mechanism

  • ComK: activation, PubMed, in comK regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Other regulations

• ComN: unknown, PubMed

Biological materials

Mutant

• BKE25580 (comEB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTTGTTCCCTCCAAATG,  downstream forward: _UP4_CTTTTCACGAGCTACGTGTG
• BKK25580 (comEB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTTGTTCCCTCCAAATG,  downstream forward: _UP4_CTTTTCACGAGCTACGTGTG

References

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