cdd

168

cytidine deaminase

Locus: BSU_25300

Molecular weight: 14.71 kDa

Isoelectric point: 4.7

Protein length: 136 aa

Gene length: 411 bp

Function: pyrimidine interconversion

Product: cytidine deaminase

Essential: no

E.C.: 3.5.4.5

Synonyms: cdd

Genomic Context

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List of homologs in different organisms, belongs to COG0295 (Galperin et al., 2021)

Gene

Coordinates: 2,610,927  2,611,337

The protein

Catalyzed reaction/ biological activity

• cytidine + H⁺ + H₂O --> NH₄⁺ + uridine (according to UniProt)
• 2'-deoxycytidine + H⁺ + H₂O --> 2'-deoxyuridine + NH₄⁺ (according to UniProt)

Protein family

• Cytidine and deoxycytidylate deaminase family (according to UniProt)

Domains

• CMP/dCMP-type deaminase domain (aa 1–128) (according to UniProt)

Cofactors

• Zn²⁺ PubMed

Structure

• 1JTK (PDB) (complex with inhibitor) PubMed
• 1UWZ (PDB) (with Arg56Ala substitution) PubMed
• 1UX0 (PDB) (with Arg56Gln substitution) PubMed
• 1UX1 (PDB) (with Cys53His and Arg56Gln substitutions) PubMed
• P19079 (AlphaFold)

Expression and Regulation

Operons

• Genes: cdd-era-yqzL-recO

  Description: PubMed

  Expression

  • expression is increased upon depletion of depletion of tRNA maturation factors rnpB or rnz PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigB: sigma factor, PubMed, in sigB regulon

Biological materials

Mutant

• BKE25300 (cdd::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGTACATTCCTCTCTT,  downstream forward: _UP4_GGCGCATTTTCATCGGAGGA
• BKK25300 (cdd::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGTACATTCCTCTCTT,  downstream forward: _UP4_GGCGCATTTTCATCGGAGGA

References

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