besA

168

Ferri-bacillibactin esterase

Locus: BSU_32010

Molecular weight: 35.34 kDa

Isoelectric point: 8.2

Protein length: 289 aa

Gene length: 870 bp

Function: iron acquisition

Product: ferri-bacillibactin esterase

Essential: no

Synonyms: besA, yuiI

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2819 (Galperin et al., 2021)

Gene

Coordinates: 3,292,490  3,293,359

The protein

Catalyzed reaction/ biological activity

• catalyses ferri-bacillibactin hydrolysis leading to cytosolic iron release PubMed

Protein family

• Esterase D family (single member, according to UniProt)

Structure

• 2QM0 (PDB) (from B. cereus, 46% identity)
• O32102 (AlphaFold)

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: besA

  Description: PubMed

  Regulation

  • induced upon iron starvation (Fur) PubMed

  Regulatory mechanism

  • Fur: repression, PubMed, in fur regulon
  • AbrB: repression, PubMed, in abrB regulon

  Additional information

  • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

Mutant

• MGNA-A650 (yuiI::erm), available at the NBRP B. subtilis, Japan
• BKE32010 (besA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCAGTACGACATTCCTC,  downstream forward: _UP4_TGAGACATACTCAGCCTTGC
• BKK32010 (besA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCAGTACGACATTCCTC,  downstream forward: _UP4_TGAGACATACTCAGCCTTGC

References

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