asnH

168

asparagine synthetase (glutamine-hydrolysing)

Locus: BSU_39920

Molecular weight: 85.66 kDa

Isoelectric point: 5.53

Protein length: 747 aa

Gene length: 2244 bp

Function: biosynthesis of asparagine

Product: asparagine synthetase (glutamine-hydrolysing)

Essential: no

E.C.: 6.3.5.4

Synonyms: asnH, yxaN

Genomic Context

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List of homologs in different organisms, belongs to COG0367 (Galperin et al., 2021)

Gene

Coordinates: 4,098,926  4,101,169

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + L-aspartate + L-glutamine --> AMP + diphosphate + H⁺ + L-asparagine + L-glutamate (according to UniProt)

Protein family

• asparagine synthetase family (with AsnB and AsnO, according to UniProt)

Domains

• Glutamine amidotransferase type-2 domain (aa 2-218) (according to UniProt)

Structure

• 1CT9 (PDB) (from E. coli, corresponds to aa 30 ... 537, 27% identity) PubMed
• P42113 (AlphaFold)

Expression and Regulation

Operons

• Genes: yxbB-yxbA-yxnB-asnH-yxaM

  Description: PubMed

  Regulation

  • repressed by glucose (8-fold) PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, in codY regulon
  • AbrB: repression, PubMed, in abrB regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• GP4146 (asnH::ermC), available in  Jörg Stülke's lab
• MGNA-B689 (asnH::erm), available at the NBRP B. subtilis, Japan
• BKE39920 (asnH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGTCTCCCTCCCAATA,  downstream forward: _UP4_AATCTGCCAGAAGGAGCATA
• BKK39920 (asnH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGTCTCCCTCCCAATA,  downstream forward: _UP4_AATCTGCCAGAAGGAGCATA

References

Reviews

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Original Publications

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