pckA
168
phosphoenolpyruvate carboxykinase
Locus
BSU_30560
Molecular weight
58.13 kDa
Isoelectric point
5.12
Function
synthesis of phosphoenolpyruvate
Product
phosphoenolpyruvate carboxykinase
Essential
no
E.C.
4.1.1.49
Synonyms
pckA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1866 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,129,530 3,131,113
The protein
Catalyzed reaction/ biological activity
Protein family
phosphoenolpyruvate carboxykinase (ATP) family (single member, according to UniProt)
Nucleotide binding Domain (233240)
Structure
1J3B (PDB) (from Thermus thermophilus, 55.1% identity) PubMed
cytoplasm (according to Swiss-Prot), cytoplasm
Additional information
Expression and Regulation
Operons
Biological materials
Mutant
GP1147 (pckA::neo), available in Jörg Stülke's lab
Expression vectors
pGP1753 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Jörg Stülke's lab) PubMed
pGP1762 (for expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab)
pGP1763 (for expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab)
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
FLAG-tag construct
LacZ fusion
pGP3312 (cat, based on pAC5), available in Jörg Stülke's lab
GFP fusion
GP1430 (spc, based on pGP1870), available in Jörg Stülke's lab
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
References
Developmentally-regulated proteolysis by MdfA and ClpCP mediates metabolic differentiation during Bacillus subtilis sporulation.bioRxiv : the preprint server for biology. 2024 Nov 26; . PMID: 39651166
Protein complexes in cells by AI-assisted structural proteomics.Molecular systems biology. 2023 Feb 23; :e11544. PMID: 36815589
Malate metabolism in Bacillus subtilis: distinct roles for three classes of malate-oxidizing enzymes.
FEMS microbiology letters. 2013 Feb; 339(1):17-22. doi:10.1111/1574-6968.12041. PMID:23136871
Reconciling molecular regulatory mechanisms with noise patterns of bacterial metabolic promoters in induced and repressed states.
Proceedings of the National Academy of Sciences of the United States of America. 2012 Jan 03; 109(1):155-60. doi:10.1073/pnas.1110541108. PMID:22190493
Physical interactions between tricarboxylic acid cycle enzymes in Bacillus subtilis: evidence for a metabolon.
Metabolic engineering. 2011 Jan; 13(1):18-27. doi:10.1016/j.ymben.2010.10.001. PMID:20933603
CcpN controls central carbon fluxes in Bacillus subtilis.
Journal of bacteriology. 2008 Sep; 190(18):6178-87. doi:10.1128/JB.00552-08. PMID:18586936
Structure of ATP-dependent phosphoenolpyruvate carboxykinase from Thermus thermophilus HB8 showing the structural basis of induced fit and thermostability.Acta crystallographica. Section D, Biological crystallography. 2005 Nov; 61(Pt 11):1500-7. PMID: 16239727
CcpN (YqzB), a novel regulator for CcpA-independent catabolite repression of Bacillus subtilis gluconeogenic genes.
Molecular microbiology. 2005 Mar; 55(5):1435-51. . PMID:15720552
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metabolic engineering. 2003 Apr; 5(2):133-49. . PMID:12850135
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading, England). 1997 Nov; 143 ( Pt 11):3431-41. . PMID:9387221
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Time of last update: 2025-08-17 22:22:44
Author of last update: Jstuelk