Difference between revisions of "Ndk"

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* '''Additional information:'''
 
* '''Additional information:'''
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 2721 {{PubMed|24696501}}
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** number of protein molecules per cell (complex medium with amino acids, without glucose): 39075 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:56, 17 April 2014

  • Description: nucleoside diphosphate kinase

Gene name ndk
Synonyms
Essential no
Product nucleoside diphosphate kinase
Function maintainance of an equilibrium between the
concentrations of different nucleoside triphosphates
Gene expression levels in SubtiExpress: ndk
Metabolic function and regulation of this protein in SubtiPathways:
ndk
MW, pI 16 kDa, 5.568
Gene length, protein length 447 bp, 149 aa
Immediate neighbours cheR, hepT
Sequences Protein DNA DNA_with_flanks
Genetic context
Ndk context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Ndk expression.png




























Categories containing this gene/protein

nucleotide metabolism/ other, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU22730

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + nucleoside diphosphate = ADP + nucleoside triphosphate (according to Swiss-Prot)
  • Protein family: phosphate acetyltransferase and butyryltransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Thr-92 AND Ser-123 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1WKJ (from Thermus thermophilus, 59% identity, 82% similarity)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 2721 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 39075 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)