FabD

• Description: malonyl CoA-acyl carrier protein transacylase
  
  

Gene name	fabD
Synonyms	ylpE
Essential	yes PubMed
Product	malonyl CoA-acyl carrier protein transacylase
Function	fatty acid biosynthesis
Gene expression levels in SubtiExpress: fabD
Metabolic function and regulation of this protein in SubtiPathways: fabD
MW, pI	33 kDa, 4.515
Gene length, protein length	951 bp, 317 aa
Immediate neighbours	plsX, fabG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of lipids, essential genes

This gene is a member of the following regulons

ComA regulon, FapR regulon

The gene

Basic information

• Locus tag: BSU15900

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU15900

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein] (according to Swiss-Prot)

• Protein family: fabD family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU15900

• Structure: 1MLA (the enzyme from E. coli) PubMed

• UniProt: P71019

• KEGG entry: [3]

• E.C. number: 2.3.1.39

Expression and regulation

• Operon: fapR-plsX-fabD-fabG-acpA PubMed

• Expression browser: fabD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced if the cells experience an accumulation of malonyl-CoA (FapR) PubMed
  • expressed at high cell density (ComA) PubMed

• Regulatory mechanism:
  • ComA: transcription activation (ComA) PubMed
  • FapR: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1185 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 8683 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Stephen W White, Jie Zheng, Yong-Mei Zhang, Rock
The structural biology of type II fatty acid biosynthesis.
Annu Rev Biochem: 2005, 74;791-831
[PubMed:15952903] [WorldCat.org] [DOI] (P p)