buk

168

butyrate kinase

Locus: BSU_24070

Molecular weight: 39.61 kDa

Isoelectric point: 5.47

Protein length: 363 aa

Gene length: 1092 bp

Function: utilization of branched-chain keto acids

Product: butyrate kinase

Essential: no

E.C.: 2.7.2.7

Synonyms: buk, yqiU, bkd

Genomic Context

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Categories containing this gene/protein

Metabolism, Amino acid/ nitrogen metabolism, Utilization of amino acids, Utilization of branched-chain amino acids

List of homologs in different organisms, belongs to COG3426 (Galperin et al., 2021)

This gene is a member of the following regulons

CodY regulon, SigL regulon, BkdR regulon

Gene

Coordinates: 2,501,549  2,502,640

The protein

Catalyzed reaction/ biological activity

ATP + butanoate --> ADP + butanoyl phosphate (according to UniProt)

Protein family

acetokinase family (with AckA, according to UniProt)

Structure

1X9J (PDB) (from Thermotoga maritima, 52% identity, 69% similarity)
P54532 (AlphaFold)

Localization

cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

Genes: ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB

Description: PubMed

Regulation

induced in the presence of isoleucine or valine (BkdR) PubMed

Regulatory mechanism

BkdR: activation, PubMed, in bkdR regulon
CodY: repression, PubMed, in codY regulon

Sigma factors

SigL: sigma factor, PubMed, in sigL regulon

Biological materials

Mutant

MGNA-C378 (yqiU::erm), available at the NBRP B. subtilis, Japan
BKE24070 (buk::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCATAACCTCCAGATCAA,  downstream forward: _UP4_TAGACAGACAGGAGTGAGTC
BKK24070 (buk::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCATAACCTCCAGATCAA,  downstream forward: _UP4_TAGACAGACAGGAGTGAGTC

References

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