folE

168

GTP cyclohydrolase I, required for the initial step of queuosine synthesis (tRNA modification)

Locus: BSU_22780

Molecular weight: 21.07 kDa

Isoelectric point: 6.34

Protein length: 190 aa

Gene length: 573 bp

Function: biosynthesis of folate and queuosine

Product: GTP cyclohydrolase IA

Essential: yes

E.C.: 3.5.4.16

Synonyms: folE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0302 (Galperin et al., 2021)

Gene

Coordinates: 2,384,783  2,385,355

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• GTP + H₂O --> 7,8-dihydroneopterin 3'-triphosphate + formate + H⁺ (according to UniProt)

Protein family

• GTP cyclohydrolase I family (with QueF, according to UniProt)

Cofactors

• zinc-dependent enzyme PubMed

Structure

• 3D2O (PDB) (enzyme from Neisseria gonorrhoeae) PubMed
• P19465 (AlphaFold)

Effectors of protein activity

• the interaction with ZagA/ZTP facilitates the supply of zinc to FolE under conditions of zinc limitation, and thus enzymatic activity PubMed

Expression and Regulation

Operons

• Genes: folE-mtrB

  Description: PubMed

  Regulation

  • constitutive PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

References

Reviews

Loading

Original Publications

Loading