nadB

168

L-aspartate oxidase

Locus: BSU_27870

Molecular weight: 58.07 kDa

Isoelectric point: 6.45

Protein length: 531 aa

Gene length: 1596 bp

Function: NAD biosynthesis

Product: L-aspartate oxidase

Essential: no

E.C.: 1.4.3.16

Synonyms: nadB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0029 (Galperin et al., 2021)

Gene

Coordinates: 2,847,871 → 2,849,466

The protein

Catalyzed reaction/ biological activity

• L-aspartate + O₂ --> H₂O₂ + iminosuccinate (according to UniProt)

Protein family

• FAD-dependent oxidoreductase 2 family (with SdhA, according to UniProt)

Cofactors

• FAD (according to UniProt)

Structure

• 5KXJ (PDB) (from Salmonella typhimurium, 38% identity)
• P38032 (AlphaFold)

Modification

• phosphorylated on Arg-104 PubMed

Expression and Regulation

Operons

• Genes: nadB-nadC-nadA

  Description: PubMed

  Regulation

  • repressed in the presence of nicotinic acid (NadR) PubMed

  Regulatory mechanism

  • NadR: repression, PubMed, in nadR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • An ncRNA is predicted for 'nadB' PubMed

Biological materials

Mutant

• BKE27870 (ΔnadB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGCCATCCTCCTGTTG,  downstream forward: _UP4_AGAAAAAACGAGGGGATTTG
• BKK27870 (ΔnadB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGCCATCCTCCTGTTG,  downstream forward: _UP4_AGAAAAAACGAGGGGATTTG

Labs working on this gene/protein

• Alessandra Albertini, University of Pavia, Italy homepage

References

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