sppA

168

protease, promotes resistance to antimicrobial peptides

Locus: BSU_29530

Molecular weight: 36.52 kDa

Isoelectric point: 7.31

Protein length: 335 aa

Gene length: 1008 bp

Function: resistance to lantibiotics

Product: protease

Essential: no

E.C.: null

Synonyms: sppA, yteI

Genomic Context

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List of homologs in different organisms, belongs to COG0616 (Galperin et al., 2021)

Gene

Coordinates: 3,020,040  3,021,047

Phenotypes of a mutant

• more sensitive to lantibiotics such as nisin and subtilin PubMed
• reduced long-term survival PubMed

The protein

Catalyzed reaction/ biological activity

• serine protease that functions to cleave the remnant signal peptides left behind after protein secretion and cleavage by signal peptidases

Protein family

• peptidase S49 family (single member, according to UniProt)

Structure

• 3RST (PDB) PubMed
• O34525 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: sppA-sppI

  Description: PubMed

  Regulation

  • induced in response to cell wall stress (SigW) PubMed

  Sigma factors

  • SigW: sigma factor, PubMed, in sigW regulon

  Additional information

  • self-processes its own C-termini PubMed

Biological materials

Mutant

• MGNA-A005 (yteI::erm), available at the NBRP B. subtilis, Japan
• BKE29530 (sppA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTTCTCCTCCTTTTT,  downstream forward: _UP4_TATGCGAAGTAGGAGGGAAC
• BKK29530 (sppA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTTCTCCTCCTTTTT,  downstream forward: _UP4_TATGCGAAGTAGGAGGGAAC

References

Reviews

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Original Publications

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