bioI

168

cytochrome P450 enzyme

Locus: BSU_30190

Molecular weight: 44.70 kDa

Isoelectric point: 6.2

Protein length: 395 aa

Gene length: 1188 bp

Function: biosynthesis of biotin

Product: cytochrome P450 enzyme

Essential: no

Synonyms: bioI

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2124 (Galperin et al., 2021)

Gene

Coordinates: 3,089,226  3,090,413

The protein

Catalyzed reaction/ biological activity

• formation of pimelic acid via a multiple-step oxidative cleavage of long-chain fatty acids
• C₂-C₈-saturated long-chain fatty acyl-[ACP] + 3 O₂ + 2 reduced [flavodoxin] --> fatty aldehyde + 3 H⁺ + 3 H₂O + 2 oxidized [flavodoxin] + pimeloyl-[ACP] (according to UniProt)

Protein family

• cytochrome P450 family (according to UniProt)

Structure

• 3EJB (PDB), 3EJE (PDB) (complex with AcpA) PubMed
• P53554 (AlphaFold)

Paralogous protein(s)

• CypA, YjiB, PksS

Expression and Regulation

Operons

• Genes: bioW-bioA-bioF-bioD-bioB-bioI-ytbQ

  Description: PubMed

  Regulation

  • repressed in the presence of biotin (BirA) PubMed

  Regulatory mechanism

  • BirA: repression, PubMed, in birA regulon

Biological materials

Mutant

• GP4749 (trpC2 ΔbioI::neo), available in Jörg Stülke's lab
• MGNA-A804 (bioI::erm), available at the NBRP B. subtilis, Japan
• BKE30190 (bioI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTGATTTTCTCCTTTCT,  downstream forward: _UP4_TAAGCCTAAGAATGTGAGTG
• BKK30190 (bioI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTGATTTTCTCCTTTCT,  downstream forward: _UP4_TAAGCCTAAGAATGTGAGTG

References

Reviews

Loading

Original Publications

Loading