pdp

168

pyrimidine nucleoside phosphorylase

Locus: BSU_39400

Molecular weight: 46.04 kDa

Isoelectric point: 4.76

Protein length: 433 aa

Gene length: 1302 bp

Function: pyrimidine interconversion

Product: pyrimidine nucleoside phosphorylase

Essential: no

E.C.: 2.4.2.2

Synonyms: pdp

Genomic Context

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List of homologs in different organisms, belongs to COG0213 (Galperin et al., 2021)

Gene

Coordinates: 4,049,009 → 4,050,310

The protein

Catalyzed reaction/ biological activity

• phosphate + uridine --> α-D-ribose 1-phosphate + uracil (according to UniProt)
• phosphate + thymidine --> 2-deoxy-α-D-ribose 1-phosphate + thymine (according to UniProt)
• 2'-deoxyuridine + phosphate --> 2-deoxy-α-D-ribose 1-phosphate + uracil (according to UniProt)

Protein family

• thymidine/pyrimidine-nucleoside phosphorylase family (single member, according to UniProt)

Structure

• 5OLN (PDB) PubMed
• P39142 (AlphaFold)

Expression and Regulation

Operons

• Genes: dra-nupC-pdp

  Description: PubMed

  Regulation

  • induced by deoxynucleotides (DeoR) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon
  • DeoR: repression, PubMed, in deoR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Additional information

• the mRNA is cleaved by RNase III PubMed

Biological materials

Mutant

• BKE39400 (Δpdp::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTGGTCACCGATCCT,  downstream forward: _UP4_TAAAAAATAAAGCACATCCC
• BKK39400 (Δpdp::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTGGTCACCGATCCT,  downstream forward: _UP4_TAAAAAATAAAGCACATCCC

References

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