HprT

• Description: trigger enzyme,hypoxanthine phosphoribosyltransferase and part of a transcription activator
  
  

Gene name	hprT
Synonyms
Essential	yes PubMed
Product	hypoxanthine phosphoribosyltransferase
Function	purine salvage and interconversion, control of ftsH expression
Gene expression levels in SubtiExpress: hprT
Interactions involving this protein in SubtInteract: HprT
Metabolic function and regulation of this protein in SubtiPathways: hprT
MW, pI	20 kDa, 4.603
Gene length, protein length	540 bp, 180 aa
Immediate neighbours	tilS, ftsH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, transcription factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y), essential genes

This gene is a member of the following regulons

SigM regulon

The TilS-HprT regulon

• ftsH

The gene

Basic information

• Locus tag: BSU00680

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU00680

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)

• Protein family: purine/pyrimidine phosphoribosyltransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • inhibition of enzymatic activity by (p)ppGpp during the ´stringent response´PubMed

• Interactions:
  • TilS-HprT, the complex activates transcription of ftsH PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU00680

• Structure: 1J7J (from Salmonella typhimurium, 51% identity, 73% similarity)

• UniProt: P37472

• KEGG entry: [2]

• E.C. number: 2.4.2.8

Expression and regulation

• Operon: tilS-hprT-ftsH PubMed

• Expression browser: hprT PubMed

• Sigma factor: SigM PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1240 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 5379 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ta-Hui Lin, Yi-Nei Hu, Gwo-Chyuan Shaw
Two enzymes, TilS and HprT, can form a complex to function as a transcriptional activator for the cell division protease gene ftsH in Bacillus subtilis.
J Biochem: 2014, 155(1);5-16
[PubMed:24001521] [WorldCat.org] [DOI] (I p)

Allison Kriel, Alycia N Bittner, Sok Ho Kim, Kuanqing Liu, Ashley K Tehranchi, Winnie Y Zou, Samantha Rendon, Rui Chen, Benjamin P Tu, Jue D Wang
Direct regulation of GTP homeostasis by (p)ppGpp: a critical component of viability and stress resistance.
Mol Cell: 2012, 48(2);231-41
[PubMed:22981860] [WorldCat.org] [DOI] (I p)

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)