yqfL

168

Modulator of CcpN activity, similar to phosphoenolpyruvate synthetase regulatory protein

Locus: BSU_25240

Molecular weight: 30.20 kDa

Isoelectric point: 5.78

Protein length: 270 aa

Gene length: 813 bp

Function: inhibits CcpN activity

Product: modulator of CcpN activity

Essential: no

E.C.: 2.7.-.-

Synonyms: yqfL

Genomic Context

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List of homologs in different organisms, belongs to COG1806 (Galperin et al., 2021)

Gene

Coordinates: 2,604,121 → 2,604,933

The protein

Catalyzed reaction/ biological activity

• negative effector of CcpN activity PubMed
• ADP + N^τ-phospho-L-histidyl/L-threonyl-[pyruvate, phosphate dikinase] --> AMP + H⁺ + N^τ-phospho-L-histidyl/O-phospho-L-threonyl-[pyruvate, phosphate dikinase] (according to UniProt)
• H⁺ + N^τ-phospho-L-histidyl/O-phospho-L-threonyl-[pyruvate, phosphate dikinase] + phosphate --> diphosphate + N^τ-phospho-L-histidyl/L-threonyl-[pyruvate, phosphate dikinase] (according to UniProt)

Protein family

• pyruvate, phosphate/water dikinase regulatory protein family (single member, according to UniProt)

Domains

• nucleotide binding domain (ATP) (151–158)

Structure

• 5D0N (PDB) (from maize, 39% identity) PubMed
• P54470 (AlphaFold)

Expression and Regulation

Operons

• Genes: ccpN-yqfL

  Description: PubMed

  Regulation

  • constitutive PubMed

  Additional information

  • the intracellular concentration of CcpN is about 4 myM (according to PubMed).

Biological materials

Mutant

• GP1677 (ΔyqfL::aphA3 trpC2), available in Jörg Stülke's lab
• GP1678 (Δ(ccpN-yqfL::aphA3 trpC2), available in Jörg Stülke's lab
• MGNA-C492 (yqfL::erm), available at the NBRP B. subtilis, Japan
• BKE25240 (ΔyqfL::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTATCCCCCCGTTAGT,  downstream forward: _UP4_TAACTCAGGACGCTCTATCC
• BKK25240 (ΔyqfL::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTATCCCCCCGTTAGT,  downstream forward: _UP4_TAACTCAGGACGCTCTATCC

Expression vectors

• pGP2197, expression with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab

Two-hybrid system

• available in Jörg Stülke's lab

Labs working on this gene/protein

• Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

References

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