SecDF

• Description: preprotein translocase subunit (ATPase)
  
  

Gene name	secDF
Synonyms	secF, secD
Essential	no
Product	preprotein translocase subunit (ATPase)
Function	protein secretion
Gene expression levels in SubtiExpress: secDF
Metabolic function and regulation of this protein in SubtiPathways: SecDF
MW, pI	81 kDa, 9.519
Gene length, protein length	2211 bp, 737 aa
Immediate neighbours	yrvC, comN
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein secretion, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

• Locus tag: BSU27650

Database entries

• BsubCyc: BSU27650

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • membrane PubMed

Database entries

• BsubCyc: BSU27650

• Structure:

• UniProt: O32047

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: secDF PubMed

• Sigma factor: SigM PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 407 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1676 PubMed

Biological materials

• Mutant:
  • SM-GN3 (secDF-spc), available in Anne Galinier's and Boris Görke's labs

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)

Aileen Rubio, Xin Jiang, Kit Pogliano
Localization of translocation complex components in Bacillus subtilis: enrichment of the signal recognition particle receptor at early sporulation septa.
J Bacteriol: 2005, 187(14);5000-2
[PubMed:15995216] [WorldCat.org] [DOI] (P p)