YwlF

• Description: ribose-5-phosphate isomerase
  
  

Gene name	ywlF
Synonyms	ipc-32d
Essential	no
Product	ribose-5-phosphate isomerase
Function	pentose phosphate pathway
Gene expression levels in SubtiExpress: ywlF
Metabolic function and regulation of this protein in SubtiPathways: ywlF
MW, pI	16 kDa, 5.416
Gene length, protein length	447 bp, 149 aa
Immediate neighbours	ywlG, ywlE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

• Locus tag: BSU36920

Database entries

• BsubCyc: BSU36920

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: lacAB/rpiB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information: Reversible Mass Action Kinetics PubMed PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • Inhibited by 6-phosphogluconate and AMP PubMed
  • Inhibited by divalent ions such as Ag2+, Mg2+, Co2+ and Zn2+ PubMed
  • Activated by EDTA and 2-mercaptoethanol PubMed

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU36920

• Structure: 3HEE (from Clostridium thermocellum, 55% identity, 72% similarity)

• UniProt: P39156

• KEGG entry: [3]

• E.C. number: 5.3.1.6

Additional information

The enzyme has probably more than one active site, but with no cooperativity described PubMed

Expression and regulation

• Operon: ywlF-ywlG PubMed

• Expression browser: ywlF PubMed

• Sigma factor:

• Regulation:
  • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

• Regulatory mechanism:
  • TnrA: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 367 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1467 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

• FLAG-tag construct: GP1404 (spc, based on pGP1331), available in the Stülke lab