pbpD
168

class A penicillin-binding protein 4

Locus

BSU_31490

Molecular weight

70.45 kDa

Isoelectric point

9.31

Protein length

624 aaSequenceBlast

Gene length

1875 bpSequenceBlast

Function

bifunctional glucosyltransferase/ transpeptidase

Product

penicillin-binding protein 4

Essential

Synonyms

pbpD

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

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Categories containing this gene/protein

Cellular processes, Cell envelope and cell division, Cell wall synthesis, Penicillin-binding proteins

Groups of genes, Membrane proteins

List of homologs in different organisms, belongs to COG0744 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

3,233,911 → 3,235,785

Phenotypes of a mutant

reduced growth, morphological changes PubMed

a strain lacking all four class A penicillin-binding proteins (ponA pbpD pbpF pbpG) is severely inhibited for L-form switching in the presence of D-cycloserine PubMed

The protein

Catalyzed reaction/ biological activity

highly active transpeptidase, responsible for about 50% of D-amino acid incorporation PubMed

[GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)]_(n)-diphospho-di-trans,octa-cis-undecaprenol + β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol --> [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)]_(n+1)-diphospho-di-trans-octa-cis-undecaprenol + di-trans,octa-cis-undecaprenyl diphosphate + H⁺ (according to UniProt)

Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine (according to UniProt)

Protein family

N-terminal part: glycosyltransferase 51 family (according to UniProt)

C-terminal part: transpeptidase family (according to UniProt)

Structure

3VMA (PDB) (PBP1B from E. coli, 28% identity) PubMed

P40750 (AlphaFold)

Paralogous protein(s)

PbpF, PbpG, PonA

Localization

cell membrane (according to UniProt)

during vegetative growth: septal, distinct spots at periphery PubMed

Additional information