fpbQ

168

petrobactin (3.4-catecholate siderophore) ABC transporter (binding protein), major component of the secretome

Locus: BSU_03830

Molecular weight: 34.64 kDa

Isoelectric point: 6.63

Protein length: 317 aa

Gene length: 954 bp

Function: acquisition of iron

Product: petrobactin ABC transporter  (binding protein)

Essential: no

Synonyms: fpbQ, yclQ, fpiA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG4607 (Galperin et al., 2021)

Gene

Coordinates: 435,036 → 435,989

The protein

Catalyzed reaction/ biological activity

• uptake of the siderophore petrobactin PubMed

Protein family

• Bacterial solute-binding protein 8 family (according to UniProt)

Domains

• Fe/B12 periplasmic-binding domain (aa 56-317) (according to UniProt)

Structure

• 3GFV (PDB)
• P94421 (AlphaFold)

Localization

• associated to the membrane (via FpbN-FpbO) PubMed
• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: fpbN-fpbO-fpbP-fpbQ

  Description: PubMed

  Regulation

  • immediately induced upon iron starvation (first wave to allow iron uptake) (Fur) PubMed

  Regulatory mechanism

  • Fur: repression, PubMed, in fur regulon

Additional information

• the mRNA is cleaved by RNase III PubMed

Biological materials

Mutant

• MGNA-C067 (yclQ::erm), available at the NBRP B. subtilis, Japan
• BKE03830 (ΔfpbQ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTCCTCACCTCTTCTA,  downstream forward: _UP4_TAAAACCAAAAAGAGCCTCC
• BKK03830 (ΔfpbQ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTCCTCACCTCTTCTA,  downstream forward: _UP4_TAAAACCAAAAAGAGCCTCC

References

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