LysA

• Description: diaminopimelate decarboxylase
  
  

Gene name	lysA
Synonyms
Essential	no
Product	diaminopimelate decarboxylase
Function	biosynthesis of lysine
Gene expression levels in SubtiExpress: lysA
Metabolic function and regulation of this protein in SubtiPathways: lysA
MW, pI	48 kDa, 5.007
Gene length, protein length	1317 bp, 439 aa
Immediate neighbours	ypuA, spoVAF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, sporulation proteins

This gene is a member of the following regulons

SigG regulon, SpoVT regulon

The gene

Basic information

• Locus tag: BSU23380

Phenotypes of a mutant

• auxotrophic for lysine PubMed

Database entries

• BsubCyc: BSU23380

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Meso-2,6-diaminoheptanedioate = L-lysine + CO₂ (according to Swiss-Prot)

• Protein family: Orn/Lys/Arg decarboxylase class-II family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU23380

• Structure: 1HKW (from Mycobacterium tuberculosis, 42% identity, 61% similarity) PubMed

• UniProt: P23630

• KEGG entry: [3]

• E.C. number: 4.1.1.20

Expression and regulation

• Operon: spoVAA-spoVAB-spoVAC-spoVAD-spoVAEB-spoVAEA-spoVAF-lysA PubMed

• Expression browser: lysA PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed late during sporulation in the forespore (SigG, SpoVT) PubMed

• Regulatory mechanism:
  • SpoVT: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 627 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 2286 PubMed

Biological materials

• Mutant:
  • 1A615 ( lysA::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Alain Brans, Patrice Filée, Andy Chevigné, Aurore Claessens, Bernard Joris
New integrative method to generate Bacillus subtilis recombinant strains free of selection markers.
Appl Environ Microbiol: 2004, 70(12);7241-50
[PubMed:15574923] [WorldCat.org] [DOI] (P p)

I Bagyan, J Hobot, S Cutting
A compartmentalized regulator of developmental gene expression in Bacillus subtilis.
J Bacteriol: 1996, 178(15);4500-7
[PubMed:8755877] [WorldCat.org] [DOI] (P p)

V Azevedo, A Sorokin, S D Ehrlich, P Serror
The transcriptional organization of the Bacillus subtilis 168 chromosome region between the spoVAF and serA genetic loci.
Mol Microbiol: 1993, 10(2);397-405
[PubMed:7934830] [WorldCat.org] [DOI] (P p)

B Moldover, P J Piggot, M D Yudkin
Identification of the promoter and the transcriptional start site of the spoVA operon of Bacillus subtilis and Bacillus licheniformis.
J Gen Microbiol: 1991, 137(3);527-31
[PubMed:1903432] [WorldCat.org] [DOI] (P p)