yusI

168

similar to arsenate reductase, binds to DNA lesions, may be involved in DNA repair

Locus: BSU_32810

Molecular weight: 13.77 kDa

Isoelectric point: 5.59

Protein length: 118 aa

Gene length: 357 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yusI

Genomic Context

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List of homologs in different organisms, belongs to COG1393 (Galperin et al., 2021)

Gene

Coordinates: 3,366,573  3,366,929

The protein

Catalyzed reaction/ biological activity

• binds to RNA base DNA lesions PubMed

Protein family

• ArsC family (with MgsR and Spx, according to UniProt)

Structure

• 2M46 (PDB) (from Staphylococcus aureus, 51% identity)
• O32175 (AlphaFold)

Expression and Regulation

Operons

• Genes: yusI-gcvH-yusG

  Description: PubMed

Biological materials

Mutant

• MGNA-B593 (yusI::erm), available at the NBRP B. subtilis, Japan
• BKE32810 (yusI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTCTTCCCCCTCAATA,  downstream forward: _UP4_TAATGTAAATTTTTTTTGAA
• BKK32810 (yusI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTCTTCCCCCTCAATA,  downstream forward: _UP4_TAATGTAAATTTTTTTTGAA

Original Publications

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