tyrA

168

prephenate dehydrogenase

Locus: BSU_22610

Molecular weight: 41.28 kDa

Isoelectric point: 5.47

Protein length: 371 aa

Gene length: 1116 bp

Function: biosynthesis of tyrosine

Product: prephenate dehydrogenase

Essential: no

E.C.: 1.3.1.12

Synonyms: tyrA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0287 (Galperin et al., 2021)

Gene

Coordinates: 2,369,251 → 2,370,366

The protein

Catalyzed reaction/ biological activity

• Prephenate + NAD⁺ --> 4-hydroxyphenylpyruvate + CO₂ + NADH (according to UniProt)

Protein family

• prephenate/arogenate dehydrogenase family (single member, according to UniProt)

Domains

• Prephenate/arogenate dehydrogenase domain (aa 6-295) (according to UniProt)
• ACT domain (aa 300-371) (according to UniProt)

Cofactors

• NAD⁺ (according to UniProt)

Structure

• 3DZB (PDB) (from Streptococcus thermophilus, 42% identity)
• P20692 (AlphaFold)

Effectors of protein activity

• subject to feedback inhibition by tyrosine PubMed

Expression and Regulation

Operons

• Genes: trpE-trpD-trpC-trpF-trpB-trpA-hisC-tyrA-aroE

  Description: PubMed

  Regulation

  • not expressed if tryptophan is available (MtrB) PubMed
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

  Regulatory mechanism

  • MtrB: transcription termination, PubMed, in mtrB regulon

• Genes: hisC-tyrA-aroE

  Description: PubMed

  Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

Mutant

• GP2358 ∆''tyrA''::kan, available in Jörg Stülke's lab
• GP4791 ∆''tyrA''::neo, available in Jörg Stülke's lab
• BKE22610 (ΔtyrA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACGTCATCACCTGGTTT,  downstream forward: _UP4_TTTTATGCTGATTGAGGTGG
• BKK22610 (ΔtyrA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACGTCATCACCTGGTTT,  downstream forward: _UP4_TTTTATGCTGATTGAGGTGG

References

Reviews

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Original Publications

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