IleS

• Description: isoleucyl-tRNA synthetase
  
  

Gene name	ileS
Synonyms
Essential	yes PubMed
Product	isoleucyl-tRNA synthetase
Function	translation
Gene expression levels in SubtiExpress: ileS
Metabolic function and regulation of this protein in SubtiPathways: ileS
MW, pI	104 kDa, 5.191
Gene length, protein length	2763 bp, 921 aa
Immediate neighbours	divIVA, ylyA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU15430

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU15430

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile) (according to Swiss-Prot)

• Protein family: IleS type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU15430

• Structure: 1QU2 (from Staphylococcus aureus, 61% identity) PubMed

• UniProt: Q45477

• KEGG entry: [2]

• E.C. number: 6.1.1.5

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon:

• Expression browser: ileS PubMed

• Sigma factor:

• Regulation:
  • induced by isoleucine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 2829 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 9345 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: