yqeL

yqeL
168

ribosomal silencing factor, dissociates the vacant 70S ribosomes during nutrient limitation

Locus
BSU_25620
Molecular weight
13.16 kDa
Isoelectric point
4.37
Protein length
Gene length
Function
translation
Product
ribosomal silencing factor
Essential
no
Synonyms
yqeL, rsfS

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0799 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,642,841 → 2,643,197
The protein
Catalyzed reaction/ biological activity
silences translation by binding to the L14 protein of the large ribosomal subunit and, as a consequence, impairs subunit joining  PubMed
dissociates the vacant 70S ribosomes during nutrient limitation BioRxiv
Protein family
Iojap/RsfS family (single member, according to UniProt)
Structure
2O5A (PDB) (from B. halodurans, 65% identity)
RplN-YqeL PubMed, the interaction serves to silence translation PubMed
Additional information
the protein is enriched in 45S assembly intermediates that accumulate upon depletion of RbgA PubMed
Expression and Regulation
Operons
Description
Expression
expression is increased upon depletion of depletion of tRNA maturation factors rnpB or rnz PubMed
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pgpPyqeM

2025-06-15 15:05:34

Jstuelk

156

16D917C7583868297F7E4E92450A5FA00BB1FF6D

20160DA9FBD3CC875D87545DE18F83BF8B1D0550

Biological materials
Mutant
MGNA-C500 (yqeL::erm), available at the NBRP B. subtilis, Japan
BKE25620 (ΔyqeL::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_GTTCATTCACAAATTCCTCC,  downstream forward: _UP4_GATCTTGACTTTGGAATGAA
BKK25620 (ΔyqeL::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_GTTCATTCACAAATTCCTCC,  downstream forward: _UP4_GATCTTGACTTTGGAATGAA
References
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Time of last update: 2025-06-13 23:10:13

Author of last update: Jstuelk