yerH

168

lipoprotein involved in cell division

locus: BSU_06630

Molecular weight: 44.36 kDa

pI: 4.88

Protein length: 396 aa

Gene length: 1191 bp

function: unknown

product: unknown

essential: no

synonyms: yerH

List of homologs in different organisms, belongs to COG4851 (Galperin et al., 2021)

Gene

Coordinates: 723,635  724,825

Phenotypes of a mutant

• inactivation suppresses the essentiality of [gene|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl] [pubmed|41045937]
• an [gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA] [gene|ADAE6B5C301959D90592A6F26DFE1690BF6F5552|yerH] double mutant has a severe growth defect [pubmed|41045937]

The protein

Structure

• [PDB|4HN3] (from Listeria monocytogenes, 45% identity)
• [AF|O34629]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|0BA7EC658F5C2397A90B68AC234D04966966561C|pcrB]-[gene|C73E52D214E24F21696973B19B0A44CE785D6FBA|pcrA]-[gene|03EFF93C1B0CE075AE068ECC4EDE9BE5D03C857E|ligA]-[gene|ADAE6B5C301959D90592A6F26DFE1690BF6F5552|yerH]

  description: [Pubmed|9701819]

  regulation

  • induced by DNA damage ([protein|search|LexA]) [Pubmed|16267290]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|16267290], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]

Biological materials

Mutant

• MGNA-B445 (yerH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1444 NBRP B. subtilis, Japan]
• BKE06630 ([gene|ADAE6B5C301959D90592A6F26DFE1690BF6F5552|yerH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06630 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTTTTTCAATAGAAAACACT,  downstream forward: _UP4_TGATAGAAAACCCTTGTGCC
• BKK06630 ([gene|ADAE6B5C301959D90592A6F26DFE1690BF6F5552|yerH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06630 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTTTTTCAATAGAAAACACT,  downstream forward: _UP4_TGATAGAAAACCCTTGTGCC

References

• 16267290,9701819,41045937