Difference between revisions of "QueC"

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* '''Additional information:'''
 
* '''Additional information:'''
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium): 158 {{PubMed|24696501}}
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** number of protein molecules per cell (complex medium with amino acids, without glucose): 469 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:43, 17 April 2014

  • Description: synthesis of the modified ribonucleotide queuosine

Gene name queC
Synonyms ykvJ
Essential no
Product unknown
Function tRNA modification
Gene expression levels in SubtiExpress: queC
MW, pI 24 kDa, 5.147
Gene length, protein length 657 bp, 219 aa
Immediate neighbours ykvI, queD
Sequences Protein DNA DNA_with_flanks
Genetic context
YkvJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
QueC expression.png















Categories containing this gene/protein

translation

This gene is a member of the following regulons

preQ1 riboswitch

The gene

Basic information

  • Locus tag: BSU13720

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: queC family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 158 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 469 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Zhou Gong, Yunjie Zhao, Changjun Chen, Yong Duan, Yi Xiao
Insights into ligand binding to PreQ1 Riboswitch Aptamer from molecular dynamics simulations.
PLoS One: 2014, 9(3);e92247
[PubMed:24663240] [WorldCat.org] [DOI] (I e)

Krishna C Suddala, Arlie J Rinaldi, Jun Feng, Anthony M Mustoe, Catherine D Eichhorn, Joseph A Liberman, Joseph E Wedekind, Hashim M Al-Hashimi, Charles L Brooks, Nils G Walter
Single transcriptional and translational preQ1 riboswitches adopt similar pre-folded ensembles that follow distinct folding pathways into the same ligand-bound structure.
Nucleic Acids Res: 2013, 41(22);10462-75
[PubMed:24003028] [WorldCat.org] [DOI] (I p)

Qi Zhang, Mijeong Kang, Robert D Peterson, Juli Feigon
Comparison of solution and crystal structures of preQ1 riboswitch reveals calcium-induced changes in conformation and dynamics.
J Am Chem Soc: 2011, 133(14);5190-3
[PubMed:21410253] [WorldCat.org] [DOI] (I p)

Jun Feng, Nils G Walter, Charles L Brooks
Cooperative and directional folding of the preQ1 riboswitch aptamer domain.
J Am Chem Soc: 2011, 133(12);4196-9
[PubMed:21375305] [WorldCat.org] [DOI] (I p)

Reid M McCarty, Arpád Somogyi, Guangxin Lin, Neil E Jacobsen, Vahe Bandarian
The deazapurine biosynthetic pathway revealed: in vitro enzymatic synthesis of PreQ(0) from guanosine 5'-triphosphate in four steps.
Biochemistry: 2009, 48(18);3847-52
[PubMed:19354300] [WorldCat.org] [DOI] (I p)

Mijeong Kang, Robert Peterson, Juli Feigon
Structural Insights into riboswitch control of the biosynthesis of queuosine, a modified nucleotide found in the anticodon of tRNA.
Mol Cell: 2009, 33(6);784-90
[PubMed:19285444] [WorldCat.org] [DOI] (I p)

Nenad Cicmil, Raven H Huang
Crystal structure of QueC from Bacillus subtilis: an enzyme involved in preQ1 biosynthesis.
Proteins: 2008, 72(3);1084-8
[PubMed:18491386] [WorldCat.org] [DOI] (I p)

Adam Roth, Wade C Winkler, Elizabeth E Regulski, Bobby W K Lee, Jinsoo Lim, Inbal Jona, Jeffrey E Barrick, Ankita Ritwik, Jane N Kim, Rüdiger Welz, Dirk Iwata-Reuyl, Ronald R Breaker
A riboswitch selective for the queuosine precursor preQ1 contains an unusually small aptamer domain.
Nat Struct Mol Biol: 2007, 14(4);308-17
[PubMed:17384645] [WorldCat.org] [DOI] (P p)

John S Reader, David Metzgar, Paul Schimmel, Valérie de Crécy-Lagard
Identification of four genes necessary for biosynthesis of the modified nucleoside queuosine.
J Biol Chem: 2004, 279(8);6280-5
[PubMed:14660578] [WorldCat.org] [DOI] (P p)