fecC

168

iron/ citrate ABC transporter (binding protein)

Locus: BSU_07520

Molecular weight: 34.87 kDa

Isoelectric point: 7.88

Protein length: 315 aa

Gene length: 948 bp

Function: iron uptake

Product: iron/ citrate ABC transporter (binding protein)

Essential: no

Synonyms: fecC, yfmC

Genomic Context

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List of homologs in different organisms, belongs to COG4594 (Galperin et al., 2021)

Gene

Coordinates: 825,787  826,734

Phenotypes of a mutant

• poor growth with the Fe(III)-dicitrate as single source of iron PubMed

The protein

Protein family

• Bacterial solute-binding protein 8 family (according to UniProt)

Domains

• Fe/B12 periplasmic-binding domain (aa 60-315) (according to UniProt)

Structure

• 3EIW (PDB) (from Staphylococcus aureus, the FecC-YhfQ complex, 46% identity) PubMed
• O34348 (AlphaFold)

Paralogous protein(s)

• YhfQ

Localization

• associated to the membrane (via FecD-FecE) PubMed
• extracellular (signal peptide) PubMed, lipid modification as retention signal

Expression and Regulation

Operons

• Genes: yfmB-fecC

  Description: PubMed

  Regulation

  • induced upon iron starvation (Fur) PubMed

  Regulatory mechanism

  • Fur: repression, PubMed, in fur regulon

• Genes: fecC-fecD-fecE-fecF

  Description: PubMed

  Regulation

  • immediately induced upon iron starvation (first wave to allow iron uptake) (Fur) PubMed

  Regulatory mechanism

  • Fur: repression, PubMed, in fur regulon

• Genes: fecC

  Description: PubMed

  Regulation

  • induced upon iron starvation (Fur) PubMed

Biological materials

Mutant

• MGNA-C246 (yfmC::erm), available at the NBRP B. subtilis, Japan
• BKE07520 (fecC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTCATTCTTCCTTTTTT,  downstream forward: _UP4_TAGGACAAATGAGAAGCGAG
• BKK07520 (fecC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTCATTCTTCCTTTTTT,  downstream forward: _UP4_TAGGACAAATGAGAAGCGAG

References

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