GcvPB

• Description: glycine decarboxylase (subunit 2)
  
  

Gene name	gcvPB
Synonyms	yqhK, gcvP
Essential	no
Product	glycine decarboxylase (subunit 2)
Function	glycine utilization
Gene expression levels in SubtiExpress: gcvPB
MW, pI	54 kDa, 5.283
Gene length, protein length	1464 bp, 488 aa
Immediate neighbours	yqhL, gcvPA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids, membrane proteins

This gene is a member of the following regulons

Gly-box

The gene

Basic information

• Locus tag: BSU24550

Database entries

• BsubCyc: BSU24550

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Glycine + H-protein-lipoyllysine = H-protein-S-aminomethyldihydrolipoyllysine + CO₂ (according to Swiss-Prot)

• Protein family: C-terminal subunit subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU24550

• Structure:

• UniProt: P54377

• KEGG entry: [3]

• E.C. number: 1.4.4.2

Expression and regulation

• Operon: gcvT-gcvPA-gcvPB PubMed

• Expression browser: gcvPB PubMed

• Sigma factor:

• Regulation: repressed by casamino acids PubMed , induced by glycine PubMed

• Regulatory mechanism:
  • induction requires a co-operatively acting riboswitch (Gly-box) PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1161 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 3235 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Maumita Mandal, Mark Lee, Jeffrey E Barrick, Zasha Weinberg, Gail Mitchell Emilsson, Walter L Ruzzo, Ronald R Breaker
A glycine-dependent riboswitch that uses cooperative binding to control gene expression.
Science: 2004, 306(5694);275-9
[PubMed:15472076] [WorldCat.org] [DOI] (I p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)