nfu

168

Fe-S carrier protein

Locus: BSU_32220

Molecular weight: 12.28 kDa

Isoelectric point: 4.18

Protein length: 111 aa

Gene length: 336 bp

Function: assembly of Fe-S clusters

Product: Fe-S carrier

Essential: no

Synonyms: yutI

Genomic Context

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List of homologs in different organisms, belongs to COG0694 (Galperin et al., 2021)

Gene

Coordinates: 3,310,386  3,310,721

The protein

Protein family

• NifU family (with SufU, according to UniProt)

Structure

• 1XHJ (PDB) (from Staphylococcus epidermidis, 81% identity)
• O32119 (AlphaFold)

Expression and Regulation

Operons

• Genes: nfu

  Description: PubMed

Biological materials

Mutant

• MGNA-A971 (yutI::erm), available at the NBRP B. subtilis, Japan
• BKE32220 (nfu::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCAGACTTCAACACAAA,  downstream forward: _UP4_TAATAAAGCAGCCAGGCTGA
• BKK32220 (nfu::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCAGACTTCAACACAAA,  downstream forward: _UP4_TAATAAAGCAGCCAGGCTGA

References

Reviews

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Original Publications

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