mtnN

168

methylthioadenosine nucleosidase

Locus: BSU_27270

Molecular weight: 25.12 kDa

Isoelectric point: 4.84

Protein length: 231 aa

Gene length: 696 bp

Function: methionine salvage

Product: methylthioadenosine nucleosidase

Essential: no

E.C.: 3.2.2.9

Synonyms: mtnN, yrrU, mtn, mthA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0775 (Galperin et al., 2021)

Gene

Coordinates: 2,787,130  2,787,825

The protein

Catalyzed reaction/ biological activity

• H₂O + S-adenosyl-L-homocysteine --> adenine + S-(5-deoxy-D-ribos-5-yl)-L-homocysteine (according to UniProt)
• H₂O + S-methyl-5'-thioadenosine --> adenine + S-methyl-5-thio-D-ribose (according to UniProt)

Protein family

• PNP/UDP phosphorylase family (with DeoD, according to UniProt)

Structure

• 1JYS (PDB) (from Escherichia coli, 51% identity, 70% similarity) PubMed
• O32028 (AlphaFold)

Expression and Regulation

Operons

• Genes: yrrT-mtnN-mccA-mccB-yrhC

  Description: PubMed

  Regulation

  • repressed in the presence of cysteine (CymR) PubMed

  Regulatory mechanism

  • CymR: repression, PubMed, in cymR regulon
  • Spx: activation, PubMed, in spx regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A850 (yrrU::erm), available at the NBRP B. subtilis, Japan
• BKE27270 (mtnN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTATTCACATTCCCTTC,  downstream forward: _UP4_TAAAACGGGGAGAACGTTCT
• BKK27270 (mtnN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTATTCACATTCCCTTC,  downstream forward: _UP4_TAAAACGGGGAGAACGTTCT

References

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