glpX

168

class II fructose-1,6-bisphosphatase, gluconeogenesis

Locus: BSU_37090

Molecular weight: 33.80 kDa

Isoelectric point: 5.07

Protein length: 321 aa

Gene length: 966 bp

Function: gluconeogenesis

Product: class II fructose-1,6-bisphosphatase

Essential: no

E.C.: 3.1.3.11

Synonyms: glpX, ywjI

Genomic Context

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Categories containing this gene/protein

Metabolism, Carbon metabolism, Carbon core metabolism, Gluconeogenesis

List of homologs in different organisms, belongs to COG1494 (Galperin et al., 2021)

Gene

Coordinates: 3,805,090  3,806,055

The protein

Catalyzed reaction/ biological activity

β-D-fructose 1,6-bisphosphate + H₂O --> β-D-fructose 6-phosphate + phosphate (according to UniProt)

Protein family

FBPase class 2 family (single member, according to UniProt)

Structure

3D1R (PDB)  (from E. coli, 49% identity, 68% similarity)PubMed
Q03224 (AlphaFold)

Kinetic information

K(M) for FBP: 20 M  PubMed

Expression and Regulation

Operons

Genes: murAB-glpX

Description: PubMed

Regulation

constitutive PubMed

Genes: spo0F-fbaA-ywjH-murAB-glpX-rho-rpmE

Description: PubMed

Expression

readthrough-transcription from the SigH-dependent promoter after the initiation of sporulation in the mother cell PubMed

Biological materials

Mutant

MGNA-B658 (ywjI::erm), available at the NBRP B. subtilis, Japan
BKE37090 (glpX::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCAACACGATCTCCTC,  downstream forward: _UP4_TAACAAACAGAAAAAGCGGG
BKK37090 (glpX::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCAACACGATCTCCTC,  downstream forward: _UP4_TAACAAACAGAAAAAGCGGG

References

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