glpX

168

class II fructose-1,6-bisphosphatase, gluconeogenesis

Locus: BSU_37090

Molecular weight: 33.80 kDa

Isoelectric point: 5.07

Protein length: 321 aa

Gene length: 966 bp

Function: gluconeogenesis

Product: class II fructose-1,6-bisphosphatase

Essential: no

E.C.: 3.1.3.11

Synonyms: glpX, ywjI

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1494 (Galperin et al., 2021)

Gene

Coordinates: 3,805,090  3,806,055

The protein

Catalyzed reaction/ biological activity

• β-D-fructose 1,6-bisphosphate + H₂O --> β-D-fructose 6-phosphate + phosphate (according to UniProt)

Protein family

• FBPase class 2 family (single member, according to UniProt)

Structure

• 3D1R (PDB)  (from E. coli, 49% identity, 68% similarity)PubMed
• Q03224 (AlphaFold)

Kinetic information

• K(M) for FBP: 20 M  PubMed

Expression and Regulation

Operons

• Genes: murAB-glpX

  Description: PubMed

  Regulation

  • constitutive PubMed

• Genes: spo0F-fbaA-ywjH-murAB-glpX-rho-rpmE

  Description: PubMed

  Expression

  • readthrough-transcription from the SigH-dependent promoter after the initiation of sporulation in the mother cell PubMed

Biological materials

Mutant

• MGNA-B658 (ywjI::erm), available at the NBRP B. subtilis, Japan
• BKE37090 (glpX::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCAACACGATCTCCTC,  downstream forward: _UP4_TAACAAACAGAAAAAGCGGG
• BKK37090 (glpX::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCAACACGATCTCCTC,  downstream forward: _UP4_TAACAAACAGAAAAAGCGGG

References

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