cspC

168

cold shock protein

Locus: BSU_05120

Molecular weight: 7.12 kDa

Isoelectric point: 4.52

Protein length: 66 aa

Gene length: 201 bp

Function: RNA chaperone

Product: cold shock protein

Essential: no

Synonyms: cspC

Genomic Context

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List of homologs in different organisms, belongs to COG1278 (Galperin et al., 2021)

Gene

Coordinates: 559,264 → 559,464

Phenotypes of a mutant

• cspB cspC double mutants are poorly viable at 15°C PubMed

The protein

Domains

• CSD domain (aa 4-63) (according to UniProt)

Structure

• 2I5M (PDB) (CspB, 71% identity) PubMed
• P39158 (AlphaFold)

Paralogous protein(s)

• CspD, CspB

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: cspC

  Description: PubMed

  Expression

  • expression is increased at low temperature PubMed

Biological materials

Mutant

• GP1969 (ΔcspC::spec trpC2) available in Jörg Stülke's lab PubMed
• BKE05120 (ΔcspC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTGTTGCCTCCTAGT,  downstream forward: _UP4_TAATCTTCAATCGTTTATAC
• BKK05120 (ΔcspC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTGTTGCCTCCTAGT,  downstream forward: _UP4_TAATCTTCAATCGTTTATAC

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

LacZ fusion

• GP1984 (amyE::(P_cspC-lacZ cat) trpC2) available in Jörg Stülke's lab PubMed

References

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