ylbN

168

may be required for accumulation of 23S rRNA

Locus: BSU_15070

Molecular weight: 19.85 kDa

Isoelectric point: 4.31

Protein length: 172 aa

Gene length: 519 bp

Function: accumulation of 23S rRNA

Product: unknown

Essential: no

Synonyms: ylbN

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1399 (Galperin et al., 2021)

Gene

Coordinates: 1,575,264 → 1,575,782

The protein

Domains

• DUF177, aa 56-165

Structure

• O34445 (AlphaFold)

Modification

• phosphorylated on Arg-158 PubMed

Expression and Regulation

Operons

• Genes: ylbN-rpmF

  Description: PubMed

  Regulation

  • strongly repressed in response to glucose starvation in M9 medium PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

Biological materials

Mutant

• MGNA-B251 (ylbN::erm), available at the NBRP B. subtilis, Japan
• BKE15070 (ΔylbN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGTATCACCTCAGGAA,  downstream forward: _UP4_TAACTCTTTAAGGAGGTGGG
• BKK15070 (ΔylbN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGTATCACCTCAGGAA,  downstream forward: _UP4_TAACTCTTTAAGGAGGTGGG

References

Reviews

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Original Publications

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