hit

168

Hit-like protein involved in cell-cycle regulation, similar to Ap₄A hydrolase

Locus: BSU_10030

Molecular weight: 16.16 kDa

Isoelectric point: 6.13

Protein length: 145 aa

Gene length: 438 bp

Function: cell-cycle regulation

Product: Hit-like protein involved in cell-cycle regulation

Essential: no

Synonyms: hit, yhaE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0537 (Galperin et al., 2021)

Gene

Coordinates: 1,076,515 → 1,076,952

The protein

Domains

• HIT domain (aa 8-116) (according to UniProt)

Structure

• 1Y23 (PDB)
• O07513 (AlphaFold)

Expression and Regulation

Operons

• Genes: hit

  Description: PubMed

Biological materials

Mutant

• MGNA-A688 (hit::erm), available at the NBRP B. subtilis, Japan
• BKE10030 (Δhit::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAGGGTTCCTCCTTATG,  downstream forward: _UP4_TAAGATGAGGTTATTTTATT
• BKK10030 (Δhit::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAGGGTTCCTCCTTATG,  downstream forward: _UP4_TAAGATGAGGTTATTTTATT