thiE

168

thiamine-phosphate pyrophosphorylase

Locus: BSU_38290

Molecular weight: 23.53 kDa

Isoelectric point: 5.06

Protein length: 222 aa

Gene length: 669 bp

Function: biosynthesis of thiamine by 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate to yield thiamine phosphate

Product: thiamine-phosphate pyrophosphorylase

Essential: no

E.C.: 2.5.1.3

Synonyms: thiE, ipa-26d, ywbK

Genomic Context

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List of homologs in different organisms, belongs to COG0352 (Galperin et al., 2021)

Gene

Coordinates: 3,930,707  3,931,375

The protein

Catalyzed reaction/ biological activity

• 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H⁺ --> CO₂ + diphosphate + thiamine phosphate (according to UniProt)
• 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H⁺ --> CO₂ + diphosphate + thiamine phosphate (according to UniProt)
• 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 4-methyl-5-(2-phosphooxyethyl)-thiazole + H⁺ --> diphosphate + thiamine phosphate (according to UniProt)

Protein family

• thiamine-phosphate synthase family (single member, according to UniProt)

Structure

• 1G6C (PDB) PubMed
• P39594 (AlphaFold)

Paralogous protein(s)

• TenI

Expression and Regulation

Operons

• Genes: ywbI-thiM-thiE

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE38290 (thiE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCATTTCCCGAGAAATAC,  downstream forward: _UP4_TAATCGTTCATTTTCCTTCA
• BKK38290 (thiE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCATTTCCCGAGAAATAC,  downstream forward: _UP4_TAATCGTTCATTTTCCTTCA

References

Reviews

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Original Publications

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