PheT

• Description: phenylalanyl-tRNA synthetase (beta subunit)
  
  

Gene name	pheT
Synonyms
Essential	yes PubMed
Product	phenylalanyl-tRNA synthetase (beta subunit)
Function	translation
Gene expression levels in SubtiExpress: pheT
Interactions involving this protein in SubtInteract: PheT
Metabolic function and regulation of this protein in SubtiPathways: pheT
MW, pI	87 kDa, 4.864
Gene length, protein length	2412 bp, 804 aa
Immediate neighbours	rnhC, pheS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, most abundant proteins

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU28630

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU28630

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe) (according to Swiss-Prot)

• Protein family: Type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions: PheS-PheT

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU28630

• Structure: 1PYS (from Thermus thermophilus hb8 mutant, 37% identity, 56% similarity) PubMed

• UniProt: P17922

• KEGG entry: [3]

• E.C. number: 6.1.1.20

Expression and regulation

• Operon: pheS-pheT PubMed

• Expression browser: pheT PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by phenylalanine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1145 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 2843 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: