ymcB

168

tRNA methylthiotransferase

Locus: BSU_17010

Molecular weight: 58.00 kDa

Isoelectric point: 6.87

Protein length: 509 aa

Gene length: 1530 bp

Function: tRNA maturation

Product: tRNA methylthiotransferase

Essential: no

Synonyms: miaB, ymcB

Genomic Context

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List of homologs in different organisms, belongs to COG0621 (Galperin et al., 2021)

Gene

Coordinates: 1,772,843  1,774,372

The protein

Catalyzed reaction/ biological activity

• modifies N(6)-isopentenyladenosine (i(6)A) to 2-methylthio-N(6)-isopentenyladenosine (ms(2)i(6)A) in tRNA PubMed
• [sulfur carrier]-SH + AH₂ + N⁶-dimethylallyladenosine³⁷ in tRNA + 2 S-adenosyl-L-methionine --> 2-methylsulfanyl-N⁶-dimethylallyladenosine³⁷ in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H⁺ + L-methionine + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• methylthiotransferase family (with YqeV, according to UniProt)

Domains

• MTTase N-terminal domain (aa 66-184) (according to UniProt)
• TRAM domain (aa 440-503) (according to UniProt)

Cofactors

• Fe-S cluster PubMed

Structure

• 4JC0 (PDB) (from Thermotoga maritima, 33% for aa 70 ... 504) PubMed
• O31778 (AlphaFold)

Paralogous protein(s)

• YqeV

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ymcB-ricA

  Description: PubMed

Biological materials

Mutant

• GP1109 (spc), available in Jörg Stülke's lab
• BKE17010 (ymcB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTCATATTTTCTCCTTT,  downstream forward: _UP4_GGAGAAGCAATCGAGGTGAA
• BKK17010 (ymcB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTCATATTTTCTCCTTT,  downstream forward: _UP4_GGAGAAGCAATCGAGGTGAA

References

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