GtaB

• Description: UTP-glucose-1-phosphate uridylyltransferase, general stress protein
  
  

Gene name	gtaB
Synonyms
Essential	no
Product	UTP-glucose-1-phosphate uridylyltransferase
Function	biosynthesis of teichoic acid
Gene expression levels in SubtiExpress: gtaB
Metabolic function and regulation of this protein in SubtiPathways: gtaB
MW, pI	32 kDa, 4.913
Gene length, protein length	876 bp, 292 aa
Immediate neighbours	mnaA, yvzH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, lipid metabolism/ other, biosynthesis of cell wall components, general stress proteins (controlled by SigB), phosphoproteins

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

• Locus tag: BSU35670

Phenotypes of a mutant

• the inactivation of gtaB suppresses the poor and filametous growth of the yvcL zapA double mutant PubMed

Database entries

• BsubCyc: BSU35670

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose (according to Swiss-Prot)

• Protein family: UDPGP type 2 family (according to Swiss-Prot)

• Paralogous protein(s): YngB, YtdA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-76 PubMed
  • phosphorylated on ser/ thr/ tyr PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU35670

• Structure: 2E3D (from E. coli, 44% identity, 61% similarity) PubMed

• UniProt: Q05852

• KEGG entry: [3]

• E.C. number: 2.7.7.9

Expression and regulation

• Operon: gtaB PubMed

• Expression browser: gtaB PubMed

• Sigma factor: SigA PubMed, SigB PubMed

• Regulation:
  • induced by stress (SigB) PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 3693 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 8998 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Katarina Surdova, Pamela Gamba, Dennis Claessen, Tjalling Siersma, Martijs J Jonker, Jeff Errington, Leendert W Hamoen
The conserved DNA-binding protein WhiA is involved in cell division in Bacillus subtilis.
J Bacteriol: 2013, 195(24);5450-60
[PubMed:24097947] [WorldCat.org] [DOI] (I p)

Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc Natl Acad Sci U S A: 2012, 109(19);7451-6
[PubMed:22517742] [WorldCat.org] [DOI] (I p)

Alain Lévine, Françoise Vannier, Cédric Absalon, Lauriane Kuhn, Peter Jackson, Elaine Scrivener, Valérie Labas, Joëlle Vinh, Patrick Courtney, Jérôme Garin, Simone J Séror
Analysis of the dynamic Bacillus subtilis Ser/Thr/Tyr phosphoproteome implicated in a wide variety of cellular processes.
Proteomics: 2006, 6(7);2157-73
[PubMed:16493705] [WorldCat.org] [DOI] (P p)

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)

D Varón, S A Boylan, K Okamoto, C W Price
Bacillus subtilis gtaB encodes UDP-glucose pyrophosphorylase and is controlled by stationary-phase transcription factor sigma B.
J Bacteriol: 1993, 175(13);3964-71
[PubMed:8320212] [WorldCat.org] [DOI] (P p)

H M Pooley, D Paschoud, D Karamata
The gtaB marker in Bacillus subtilis 168 is associated with a deficiency in UDPglucose pyrophosphorylase.
J Gen Microbiol: 1987, 133(12);3481-93
[PubMed:2846750] [WorldCat.org] [DOI] (P p)