feuA

feuA
168

ABC transporter for the siderophores Fe-enterobactin and Fe-bacillibactin (binding protein), with YusV as ATPase

Locus
BSU_01630
Molecular weight
34.95 kDa
Isoelectric point
8.02
Protein length
Gene length
Product
ABC transporter for the siderophores Fe-enterobactin and Fe-bacillibactin (binding protein) PubMed
Essential
no
Synonyms
feuA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0614 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
182,370  183,323
Phenotypes of a mutant
severe growth inhibition upon addition of subinhibitory concentrations of mirubactin C PubMed
The protein
Protein family
Two independent non-symmetric globular domains, connected by a long alpha-helix (residues 143-164 of the mature protein).
Fe/B12 periplasmic-binding domain (aa 57-317) (according to UniProt)
Structure
2PHZ (PDB), 2WI8 (PDB), 2WHY (PDB) (complex with ferri-bacillibactin)
2XUZ (PDB) (complex with ferri-enterobactin)
2XV1 (PDB) (complex with ferric mecam)
Modification
FeuA is a lipoprotein with a N-acetyl-S-diacyl-glyceryl-cysteine structure  PubMed
associated to the membrane (lipoprotein) PubMed
extracellular (signal peptide) PubMed
Expression and Regulation
Operons
Description
Regulation
expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
induced by iron starvation (second wave to allow iron scavenging from the environment) (Fur) PubMed
Regulatory mechanism
Fur: repression, PubMed, in fur regulon
Btr: activation, in the presence of the co-activators bacillibactin or enterobactin PubMed, in btr regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
the presence of an iron-responsive element bound by CitB between ''feuA and feuB'' suggests iron-dependent regulation by CitB PubMed
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feuAybbA

2024-12-18 01:07:57

Jstuelk

106

c6ed9c562a4c7db452ebc8903a1989d0bb2f0947

A3C1B309E9C3034B9896B74BA25621D2CF69902F

Other regulations
CitB: translation control
Biological materials
Mutant
BKE01630 (feuA::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATCTATAGAGCCTCCTGTT,  downstream forward: _UP4_AACTAATTCAGAGTAGGTTT
BKK01630 (feuA::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATCTATAGAGCCTCCTGTT,  downstream forward: _UP4_AACTAATTCAGAGTAGGTTT
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
References
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516B13F337FD346B4A4A268E35D1EBABB05957E1

Page visits: 6853

Time of last update: 2024-12-21 13:34:57

Author of last update: Jstuelk