metN

168

methionine ABC transporter (ATP-binding protein)

Locus: BSU_32750

Molecular weight: 37.73 kDa

Isoelectric point: 7.3

Protein length: 341 aa

Gene length: 1026 bp

Function: methionine uptake

Product: methionine ABC transporter (ATP-binding protein)

Essential: no

E.C.: 3.6.3.-

Synonyms: metN, yusC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1135 (Galperin et al., 2021)

Gene

Coordinates: 3,363,266 → 3,364,291

The protein

Protein family

• ABC transporter superfamily (according to UniProt)

Domains

• ABC transporter domain (aa 2-241) (according to UniProt)

Structure

• 3DHW (PDB) (the MetN-MetP complex from E. coli, 44% identity) PubMed
• O32169 (AlphaFold)

Localization

• associated to the cell membrane, via MetP PubMed

Expression and Regulation

Operons

• Genes: metN-metP-metQ

  Description: PubMed

  Regulation

  • activated during growth in the presence of branched chain amino acids (CodY) PubMed
  • the mRNA is processed between metP and metQ by RNase Y, this requires the RicA-RicF-RicT complex PubMed
  • the S-box RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • S-box: RNA switch, the S-box riboswitch binds S-adenosylmethionine resulting in termination, in S-box
  • CodY: activation, PubMed, in codY regulon

Biological materials

Mutant

• MGNA-A596 (yusC::erm), available at the NBRP B. subtilis, Japan
• 1A797 ( metN::cat), PubMed, available at BGSC
• BKE32750 (ΔmetN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAGCAATCACCTGCCTTA,  downstream forward: _UP4_CAGGTGAAAGCAGAGGTGAT
• BKK32750 (ΔmetN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAGCAATCACCTGCCTTA,  downstream forward: _UP4_CAGGTGAAAGCAGAGGTGAT

References

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