infA

168

translation initiation factor IF-1, binds to 30S ribosome subunits to facilitate initiator tRNA binding

Locus: BSU_01390

Molecular weight: 8.08 kDa

Isoelectric point: 7.26

Protein length: 72 aa

Gene length: 219 bp

Function: translation

Product: initiation factor IF-1

Essential: yes

Synonyms: infA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0361 (Galperin et al., 2021)

Gene

Coordinates: 147,585 → 147,803

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• binds to 30S ribosome subunits to facilitate initiator tRNA binding PubMed

Protein family

• IF-1 family (single member, according to UniProt)

Domains

• S1-like domain (aa 2-72) (according to UniProt)

Structure

• 4QL5 (PDB) (from Streptococcus pneumoniae, 79% identity)
• P20458 (AlphaFold)

Modification

• phosphorylation on Tyr-60 PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: rpsJ-rplC-rplD-rplW-rplB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ-rplN-rplX-rplE-rpsN-rpsH-rplF-rplR-rpsE-rpmD-rplO-secY-adk-map-oapB-infA-rpmJ-rpsM-rpsK-S56-rpoA-rplQ

  Description: PubMed

  Regulation

  • strongly repressed in response to glucose starvation in M9 medium PubMed
  • likely autorepression of operon expression upon binding of excess of one ribosomal protein to the untranslated region of the mRNA PubMed
  • expression is reduced in motile cells as compared to non-motile cells PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

  Sigma factors

  • SigA: sigma factor, PubMed (two promoters, about 140bp and 200bp upstream of the start codon), in sigA regulon

  Additional information

  • term-seq has identified a potential novel regulatory RNA element (protein-dependent leader) including an intrinsic transcription terminator upstream of rpsJ PubMed

References

Reviews

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Original Publications

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