rok

168

chromosomal loop anchor, repressor of comK, nucleoid associated protein that serves to help repress expression of A T-rich genes, many of which appear to have been acquired by horizontal gene transfer

Locus: BSU_14240

Molecular weight: 21.69 kDa

Isoelectric point: 9.7

Protein length: 191 aa

Gene length: 576 bp

Function: control of genome organization, silencing of horizontally acquired genes

Product: chromosomal loop anchor

Essential: no

Synonyms: rok, ykuW

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 1,493,787  1,494,362

Phenotypes of a mutant

• higher excision rate of the conjugative transposon ICEBs1 PubMed

The protein

Catalyzed reaction/ biological activity

• binds to extended regions of the B. subtilis genome that are characterized by a high A+T content and are known or believed to have been acquired by horizontal gene transfer PubMed

Domains

• N-terminal multimerization domain PubMed
• C-terminal DNA-binding domain PubMed

Structure

• 5ZUX (PDB) (complexed with DNA) PubMed
• 5ZUZ (PDB) PubMed
• O34857 (AlphaFold)

Effectors of protein activity

• activity is modulated by interaction with DnaA PubMed

Expression and Regulation

Operons

• Genes: rok

  Description: PubMed

  Regulation

  • repressed by Rok PubMed

  Regulatory mechanism

  • Spo0A: repression, PubMed, in spo0A regulon
  • SinR: repression, PubMed, in sinR regulon
  • Rok: repression, PubMed, in rok regulon
  • AbrB: repression, PubMed, in abrB regulon

Biological materials

Mutant

• MGNA-B345 (ykuW::erm), available at the NBRP B. subtilis, Japan
• BKE14240 (rok::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCCTCAATGTACCCCCT,  downstream forward: _UP4_TAAATATAAAGAAAAACTGC
• BKK14240 (rok::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCCTCAATGTACCCCCT,  downstream forward: _UP4_TAAATATAAAGAAAAACTGC

References

Reviews

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Original Publications

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