Difference between revisions of "PheS"

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* '''Additional information:'''
 
* '''Additional information:'''
 
** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
 
** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium): 1358 {{PubMed|24696501}}
 +
** number of protein molecules per cell (complex medium with amino acids, without glucose): 2796 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:35, 17 April 2014

Gene name pheS
Synonyms
Essential yes PubMed
Product phenylalanyl-tRNA synthetase (alpha subunit)
Function translation
Gene expression levels in SubtiExpress: pheS
Interactions involving this protein in SubtInteract: PheS
Metabolic function and regulation of this protein in SubtiPathways:
pheS
MW, pI 38 kDa, 5.083
Gene length, protein length 1032 bp, 344 aa
Immediate neighbours pheT, ysgA
Sequences Protein DNA DNA_with_flanks
Genetic context
PheS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PheS expression.png















Categories containing this gene/protein

translation, essential genes, universally conserved proteins, most abundant proteins

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU28640

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe) (according to Swiss-Prot)
  • Protein family: Phe-tRNA synthetase alpha chain type 1 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 1PYS (from Thermus thermophilus hb8 mutant, 37% identity, 56% similarity) PubMed
  • KEGG entry: [3]

Additional information

universally conserved protein

Expression and regulation

  • Regulation:
  • Additional information:
    • belongs to the 100 most abundant proteins PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 1358 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 2796 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References