CcpN

• Description: transcriptional repressor of gluconeogenetic genes and of sr1. repression in the presence of glucose
  
  

Gene name	ccpN
Synonyms	yqzB
Essential	no
Product	transcriptional regulator
Function	repressor of genes involved in gluconeogenesis (gapB, pckA) and of sr1
Gene expression levels in SubtiExpress: ccpN
Interactions involving this protein in SubtInteract: CcpN
Metabolic function and regulation of this protein in SubtiPathways: ccpN
MW, pI	23.4 kDa, 7.22
Gene length, protein length	636 bp, 212 amino acids
Immediate neighbours	yqfL, glyS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, transcription factors and their control, regulators of core metabolism

The CcpN regulon:

• gapB-speD, pckA, Sr1

The gene

Basic information

• Locus tag: BSU25250

Phenotypes of a mutant

Impaired growth on glucose due to re-routing of carbon from glycolysis to the pentose phosphate pathway PubMed

Database entries

• BsubCyc: BSU25250

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription repression of the gapB, pckA, and sr1 genes in the presence of glucose PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • YqfL (negative effector of CcpN activity) PubMed
  • ATP enhances CcpN-dependent repression, ADP counteracts the repressing activity of CcpN PubMed

• Interactions:
  • active as dimer (according to PubMed)
  • CcpN-RpoA PubMed

• Localization:

Database entries

• BsubCyc: BSU25250

• Structure:

• UniProt: O34994

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ccpN-yqfL PubMed

• Expression browser: ccpN PubMed

• Sigma factor:

• Regulation: constitutively expressed PubMed

• Additional information:
  • the intracellular concentration of CcpN is about 4 myM (according to PubMed).
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 111 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 220 PubMed

Biological materials

• Mutant:
  • DB104 ccpN::cat, available in Sabine Brantl's lab
  • GP1128 ccpN::cat, available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• Antibody: available in Sabine Brantl's lab

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Sabine Brantl, Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany homepage

Uwe Sauer, ETH Zürich, Switzerland homepage

References

Reviews

Sabine Brantl, Andreas Licht
Characterisation of Bacillus subtilis transcriptional regulators involved in metabolic processes.
Curr Protein Pept Sci: 2010, 11(4);274-91
[PubMed:20408793] [WorldCat.org] [DOI] (I p)