spoVS

168

required for dehydratation of the spore core and assembly of the coat, mutation increases SigD-dependent gene expression, might act via SinR

Locus: BSU_16980

Molecular weight: 8.66 kDa

Isoelectric point: 6.47

Protein length: 86 aa

Gene length: 261 bp

Function: spore coat assembly, spore core dehydratation

Product: unknown

Essential: no

Synonyms: spoVS

Genomic Context

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List of homologs in different organisms, belongs to COG2359 (Galperin et al., 2021)

Gene

Coordinates: 1,769,935  1,770,195

Phenotypes of a mutant

• increased SigD-dependent gene expression, this might occur via SinR PubMed

The protein

Structure

• 2EK0 (PDB) (from Thermus thermophilus, 56% identity)
• P45693 (AlphaFold)

Expression and Regulation

Operons

• Genes: spoVS

  Description: PubMed

  Regulation

  • expressed at the onset of stationary phase (SigH) PubMed

  Sigma factors

  • SigH: sigma factor, PubMed, in sigH regulon

Biological materials

Mutant

• GP1601 (spoVS::cat), available in Jörg Stülke's lab
• GP1848 (ymdB-spoVS::spc cassette), available in Jörg Stülke's lab
• BKE16980 (spoVS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCTGCTCCCCCTTAGT,  downstream forward: _UP4_TAAAAACAAATAAAGCATTC
• BKK16980 (spoVS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCTGCTCCCCCTTAGT,  downstream forward: _UP4_TAAAAACAAATAAAGCATTC

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

References

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