fhuD

168

hydroxamate siderophore ABC transporter (only ferrichrome) (binding protein)

Locus: BSU_33320

Molecular weight: 34.27 kDa

Isoelectric point: 9.16

Protein length: 315 aa

Gene length: 948 bp

Function: siderophore uptake

Product: hydroxamate siderophore ABC transporter (only ferrichrome) (binding protein)

Essential: no

Synonyms: fhuD

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0614 (Galperin et al., 2021)

Gene

Coordinates: 3,418,474  3,419,421

Phenotypes of a mutant

• poor growth with the xenosiderophore ferrichrome as single source of iron PubMed

The protein

Protein family

• Bacterial solute-binding protein 8 family (according to UniProt)

Domains

• Fe/B12 periplasmic-binding domain (aa 60-315) (according to UniProt)

Structure

• 4FNA (PDB) (from Staphylococcus aureus, 38% identity) PubMed
• P37580 (AlphaFold)

Paralogous protein(s)

• YxeB

Localization

• cell membrane (according to Swiss-Prot),  extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: fhuD

  Description: PubMed

  Regulation

  • induced by iron starvation (second wave to allow iron scavenging from the environment) (Fur) PubMed

  Regulatory mechanism

  • Fur: repression, PubMed, in fur regulon

Biological materials

Mutant

• BKE33320 (fhuD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCTCATACCTTCTTTC,  downstream forward: _UP4_TAAACAAAAGAGCCGCCTGC
• BKK33320 (fhuD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCTCATACCTTCTTTC,  downstream forward: _UP4_TAAACAAAAGAGCCGCCTGC

References

Loading