trpC

168

indole-3-glycerol-phosphate synthase

Locus: BSU_22660

Molecular weight: 27.69 kDa

Isoelectric point: 5.53

Protein length: 250 aa

Gene length: 750 bp

Function: biosynthesis of tryptophan

Product: indole-3-glycerol-phosphate synthase

Essential: no

E.C.: 4.1.1.48

Synonyms: trpC

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 2,374,139 → 2,374,888

The protein

Catalyzed reaction/ biological activity

• 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H⁺ --> (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO₂ + H₂O (according to UniProt)

Protein family

• TrpC family (single member, according to UniProt)

Structure

• 1VC4 (PDB) (from Thermus thermophilus, 36% identity, 49% similarity)
• P03964 (AlphaFold)

Expression and Regulation

Operons

• Genes: trpE-trpD-trpC-trpF-trpB-trpA

  Description: PubMed

  Regulation

  • not expressed if tryptophan is available (MtrB) PubMed
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
  • the RNA switch RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • MtrB: transcription termination, PubMed, in mtrB regulon

• Genes: trpE-trpD-trpC-trpF-trpB-trpA-hisC-tyrA-aroE

  Description: PubMed

  Regulation

  • not expressed if tryptophan is available (MtrB) PubMed
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

  Regulatory mechanism

  • MtrB: transcription termination, PubMed, in mtrB regulon

Biological materials

Mutant

• BKE22660 (ΔtrpC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCAAGCATAGATCTCTTC,  downstream forward: _UP4_CATGCTTTGTTTAGGGAGTG
• BKK22660 (ΔtrpC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCAAGCATAGATCTCTTC,  downstream forward: _UP4_CATGCTTTGTTTAGGGAGTG

References

Reviews

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Original Publications

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