murAB

168

UDP-N-acetylglucosamine 1-carboxyvinyltransferase, required for efficient spore engulfment

Locus: BSU_37100

Molecular weight: 45.85 kDa

Isoelectric point: 5.83

Protein length: 429 aa

Gene length: 1290 bp

Function: peptidoglycan precursor biosynthesis

Product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase

Essential: no

E.C.: 2.5.1.7

Synonyms: murAB, murZ, lssF, rev-4

Genomic Context

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List of homologs in different organisms, belongs to COG0766 (Galperin et al., 2021)

Gene

Coordinates: 3,806,086  3,807,375

The protein

Catalyzed reaction/ biological activity

• phosphoenolpyruvate + UDP-N-acetyl-α-D-glucosamine --> phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-α-D-glucosamine(according to UniProt)

Protein family

• EPSP synthase family (with MurAA and AroE, according to UniProt)

Structure

• 3SG1 (PDB) (from B. anthracis, 50% identity, 81% similarity)
• P19670 (AlphaFold)

Paralogous protein(s)

• MurAA

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: murAB-glpX

  Description: PubMed

  Regulation

  • constitutive PubMed

• Genes: spo0F-fbaA-ywjH-murAB-glpX-rho-rpmE

  Description: PubMed

  Expression

  • readthrough-transcription from the SigH-dependent promoter after the initiation of sporulation in the mother cell PubMed

Biological materials

Mutant

• BKE37100 (murAB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATAGTCTCCTTCCATA,  downstream forward: _UP4_TAATGGTTTCTTGTGAGAGG
• BKK37100 (murAB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATAGTCTCCTTCCATA,  downstream forward: _UP4_TAATGGTTTCTTGTGAGAGG

Expression vectors

• pGP2595: (IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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